Contact detailsGeorgios A. Spyroulias, PhD.
Department of Pharmacy
University of Patras
GR-26504 Patras, GREECE
Tel: +30.2610.969950 (office)
+30.2610.969951 (terra silico)
+30.2610.969952 (terra vitro)
Your IP: 220.127.116.11
Now is: 2016-09-01 01:48
Polypeptides tend to become organized independently into a variety of functional scaffolds and to socialize with other partner molecules, forming small biomolecular complexes or large assemblies, in various intersections of biological pathways. Proteins’ architecture, plasticity and interaction determine the fate of a variety of biological events. Understanding the functional role of peptides and proteins is strongly coupled to the atomic-level insights of their structure and flexibility.
The research activities of the group encompass the study of the structure and dynamics of peptides and proteins involved in signalling pathways, proteolysis and ion/metal transfer, both in silico and in vitro.
Terra Vitro is the wet biology lab for protein production, labelling, purification and characterization. NMR study of proteins and protein complexes requires the production and isolation of the molecules labelled in 13C and 15N active NMR nuclei. Proteins with a molecular weight ranging from 8 to 22 kDa (70 to 200 amino acids) are currently over-expressed in high yields in our lab using bacterial and eukaryotic systems and purified/isolated accordingly.
The lab offers a variety of expression vectors and is equipped with all the appropriate facilities for expression, purification and analysis of recombinant native or mutated proteins, bearing a variety of purification tags.
Terra Silico is the computer lab for NMR data analysis and Biomolecular Simulations. NMR data provide an atomic-level information for the structure, dynamics, and interaction of polypeptides and can drive Structure-Activity Relationship studies through the design of protein/peptide analogues or protein substrates. Biomolecular Simulations are applied to study the conformational dynamics of protein-protein /peptide /substrate complexes.
Terra silico is equipped with powerful computational systems running software for NMR data analysis, structure calculations, docking and molecular dynamics simulation, structure validation etc.
EAST-NMR will combine activities, such as: (i) Networking, (ii) Trans-national access, and (iii) Joint research activities, in a co-ordinated manner. University of Patras is the Greek partner (Partner #12) to EAST-NMR project and is participating to a variety of activities such as, NMR workshops & conferences, young investigators meetings, etc. The program website will be available soon and will be announced.
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